Abstract
Taspine was isolated for the first time from Radix et Rhizoma Leonticis. Epidermal growth factor receptor (EGFR) is important in cell growth and differentiation and has become an important target in anti-cancer drug design. In this study, we found taspine could inhibit proliferation of A431 and HEK293/EGFR cells. To investigate whether it could enter the cell or acted on the cell membrane receptor, a cell-based assay was established to determine the concentration of taspine in A431 and HEK293/EGFR cells using high-performance liquid chromatography–mass spectrometry (HPLC–MS). The inhibitory effects of taspine on EGFR expression and mRNA expression were also investigated. The HPLC–MS results showed that taspine decreased in the supernatant liquid, and increased in the cell lysate, which indicated that taspine could enter the cell or act on the cell membrane receptor. Subsequent analysis using a cell-membrane chromatography (CMC) assay showed that taspine could act on EGFR at the cell membrane. In addition, ELISA and reverse transcriptase polymerase chain reaction (RT-PCR) assays showed that taspine inhibited proliferation, EGFR protein, and EGFR mRNA expression in A431 and HEK293/EGFR cells. These results indicated that taspine, with antitumor activity, could interact with the cell, act on EGFR at the cell membrane, and inhibit cell proliferation by down-regulating EGFR protein and mRNA expression.
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