Abstract
Abstract Algal polysaccharides exhibit a wide range of biological activities and potential applications. Many of these bioactivities correlate positively with the presence of sulfate groups on the polysaccharides. The most common method used for sulfate quantification in algal samples is the turbidimetric method using the barium chloride-gelatin reagent. However, the original procedure is difficult to adapt for routine analysis since it is laborious and time-consuming. An optimized method was established using 96-well microplates, with the advantage of reducing waste and discrimination between organic and inorganic sulfates. This proposed method produced the same accuracy as the original.
Highlights
Sulfated polysaccharides are the major structural components of the cell wall of seaweeds. These compounds exhibit a wide range of biological activities with potential medicinal, agricultural, and industrial applications (Bouissil et al, 2019; Hentati et al, 2020)
Activity studies have suggested that there can be a positive correlation between sulfate groups and the biological activities of these polysaccharides (Jiao et al, 2011)
Several methods are used to measure sulfate in samples, including protocols that require expensive and specialized equipment, such as infrared spectroscopy using the ratios of absorbance of characteristic bands
Summary
Determination of sulfate in algal polysaccharide samples: a step-by-step protocol using microplate reader. Priscila BezerraTorres1,* , Alice Nagai , Carmen Eusebia Palacios Jara , Janaína Pires Santos , Fungyi Chow , Déborah Yara Alves Cursino dos Santos
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
