Abstract

Jatropha cake was found to be best substrate for supporting the highest population dynamics and longevity of P. flourescens in vitro. Both these cakes supported the population of P. flourescens up to 120 days at 28 ± 2oC. Highest population of P. flourescens was noticed on jatrofa cake after 45 days of inoculation and incubation at 28 ± 2oC when maintained with 15% moisture, while on neem cake, highest population of P. flourescens was noticed after 60 days of inoculation when maintained with 25% moisture. Jatropha cake was better than neem cake for supporting population and longer shelf life of P. flourescens in vitro. Result of this study may help for using deoiled cakes of different TBOs (tree born oil seeds) in mass multiplication of different bio agent’s especially P. flourescens

Highlights

  • Various factors act as impediments which are known to affect the commercialization of microbial bio-control agent such as Pseudomonas fluorescens

  • Deoiled cakes of two Tree Born Oilseeds (TBOs) i.e., Neem and Jatropha Were tested for their suitability to support the population dynamics and longevity of Pseudomonas fluorescens at three different level of moisture i.e 15%, 25% and 35%

  • De-oiled cakes of two trees born oilseeds (TBOs) viz., Neem and Jatropha were tested as solid substrate for their suitability for mass multiplication of Pseudomonas flourescens Among two deoiled cakes, Jatropha cake was found to be best substrate in supporting the population dynamics and longevity as well of Pseudomonas flourescens in vitro

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Summary

Introduction

Various factors act as impediments which are known to affect the commercialization of microbial bio-control agent such as Pseudomonas fluorescens (an essential step for improving efficiency of bio-control agents). One of these impediments is the lack of technology for costeffective mass production of bio-control agents (BCAs) and their insufficient longevity during storage and transportation with a sufficient level of effective population (cfus). It is being noticed that Pseudomonas fluorescens are often effective in the laboratory, but the level of disease control, achieved in the field is sometimes disappointing and unpredictable Some of these failures can be attributed to inadequate establishment and survival of this microorganism in soil [1]. It is known to enhance plant growth along with yield enhancement and to reduce severity of many diseases [3]

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