Abstract
The amounts of sterols in vegetable oils were measured by a simple enzymatic method. The applicability of the enzymatic method to determination of various sterols was investigated.4-Desmethylsterols were oxidized quantitatively by cholesterol oxidase to give satisfactory analytical result. However, 4-mono and 4, 4-dimethylsterols could not be oxidized by cholesterol oxidase. It was also possible to analyze unsaponifiable matter in vegetable oils by this method due to the quite low content of 4-methylsterols.
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