Abstract
A simple and highly sensitive high-performance liquid chromatographic method for determining sterols in edible oils and fats is presented. Sterols were isolated from a few miligrams of sample by saponification or thin layer chromatography. They were subsequently converted into the corresponding fluorescent derivatives by heating with 9-anthroylnitrile at 100°C for 30 min in the presence of triethylamine. The derivatives were separated on a reversed phase column (Senshu pak ODS-1250-Y) with an isocratic elution using acetonitrile-dichloromethane (75/25, vol/vol). The detection limit for cholesterol was about 80 pg. The present method was shown applicable to the determination of total, free and acylated sterols in edible oils and fats with satisfactory reliability, sensitivity and handling.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
