Abstract
A method for the determination of androsterone, dehydroepiandrosterone, epiandrosterone, 5α-androstane-3α,17β-diol, androst-5-ene-3β17α-diol, androst-5-ene-3β,17β-diol, pregnenolone, pregn-5-ene-3β,20α-diol and pregn-5-ene-3β,17α,20α-triol monosulfates and androst-5-ene-3β,17α-diol, androst-5-ene-3β,17β-diol and pregn-5-ene-3β,20α-diol disulfates in human plasma is described. A plasma extract is chromatographed on Sephadex LH-20 and a monosulfate and a disulfate fraction are obtained. After solvolysis, the steroids are purified on a silicic acid column and their trimethylsilyl ethers are analyzed by gas chromatography on QF-1 and SE-30 columns. The specificity of the method was established by mass spectrometric analysis of the effluent from the GLC columns. Concentrations of steroid mono- and disulfates in peripheral plasma of a group of young healthy female and male subjects are given.
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