Abstract
We demonstrate a fully flexible micromachined cantilever-based aptasensor for sensitive and specific sensing of Staphylococcus Enterotoxin B (SEB). The proposed flexible cantilever was fabricated using parylene-C as surface passivation layers and poly(3,4-ethylenedioxythiophene)/polystyrene sulfonate (PEDOT:PSS) as strain gauge for characterization of resistance. A spring constant of prepared cantilever was measured to be 12 nN/μm together with a deflection sensitivity of 1.42×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-3</sup> /μm. With assembled 78-mer 5’-biotinylated aptamers on the sensing cantilever surface, the fully flexible aptasensor is capable of specific recognition SEB with a limit detection of 0.3 ng/mL. To evaluate the applicability of the proposed aptasensor, detections on raw milk spiked with SEB were implemented. The comparison indicates the prepared aptasensor could be qualified for SEB recognition in food contaminants detections, and the fully flexible cantilever-based sensors have tremendous potential for applications in food safety evaluation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
