Abstract
Species-specific PCR tests, based on the manganese-dependent superoxide dismutase A encoding gene ( sodA) and the chaperonin 60 encoding gene ( cpn60), were developed for the identification of Streptococcus phocae, a bacterial pathogen of seals. The selection of both oligonucleotide primer pairs was performed after amplification and sequencing of internal parts of both genes using universal oligonucleotide primers. The sequence studies of both genes additionally confirmed that S. phocae could taxonomically be classified to the pyogenic group of the genus Streptococcus.
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