Abstract
An anion exchange high speed liquid chromatographic method was presented for the simultanious separation and determination of sorbic acid, benzoic acid and saccharin in several foods. Liquid foods such as orange juice and fermented milk were injected into the chromatograph after filtration through a 0.45μm pore menbrane filter. Solid foods such as cooked bean and fish jelly products (“Kamaboko”) were extracted with borate buffer solution (pH 9.2) and filtered through menbrane filter prior to injection. Samples were eluted from a Permaphase AAX column with 10-3M sodium perchloride solution in pH 8, 10-5M borate buffer solution and were determined with a UV detector. Average recoveries of sorbic acid, benzoic acid and saccharin from the samples were 90.1 to 106.5%. However, this method have a problem that samples containing sodium chloride may result the false determination.
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