Determination of some non-steroid anti-inflammatory drugs by capillary isotachophoresis

Abstract

The isotachophoretic (ITP) behaviour and separation of the anti-inflammatory drugs kebuzone (KB), tribuzone (TB) and phenylbutazone (PB) was studied in the operational system of HCl/His (leading electrolyte, LE) and 4-nitrophenol (terminating electrolyte, TE). The effective mobilities were 19.4 × 10 −9 m 2V −1s −1 for KB, 18.1 × 10 −9 m 2V −1s −1 for TB and 18.9 × 10 −9m 2V −1s −1 for PB when using an optimised system with 10 mM HCl + 40 mM His (pH 6.63) as LE and 10 mM 4-nitrophenol as TE. The calibration graphs were rectilinear ( r = 0.9982–0.9996) in the range 20 to 600 μmol l −1 of KB, TB or PB. The ITP method was used for determining the content of KB, TB or PB in mass-produced pharmaceuticals as tablets, coated tablets, injections, and ointments. The results of the ITP determination were in good agreement with those of standard pharmacopoeial methods.