Abstract
Chitin obtained from the fermentation of Tiger Shrimp (Penaeus monodon) shell by using Providencia stuartii could be hydrolyzed into N-acetylglucosamine and another simple compound such as oligo-glucosamine. There is a limited research study regarding the isolation of oligo-glucosamine which also have a functional property. The study aimed to determine the best polar solvent and ratio sample and solvent (1:1 and 1:2, v/v) to precipitate oligo-glucosamine with the highest concentration. The polar solvents used in this study were ethanol, acetone, acetonitrile, ethanol-acetone (1:1, v/v), ethanolacetonitrile (1:1, v/v), and acetone-acetonitrile (1:1, v/v). Moreover, the yield obtained from precipitation by using those solvents were also investigated in this study. The result showed that combination of ethanol and acetonitrile (1:1, v/v) with ratio solvent-sample 1:1 (v/v) gave the highest concentration of oligo-glucosamine. The yield obtained was 0.12% (based on the volume obtained after fermentation) or 0.09% (based on the weight of chitin used for fermentation).
Highlights
Chitin from the black tiger shrimp (Penaeus monodon) shell can be hydrolyzed into its monomer and oligomer
The yield of oligoglucosamine was calculated with two different approaches as shown in these formulae: The oligo-glucosamine concentration is expressed as mg/g Triacetylchitotriose equivalent
Oligo-glucosamine less polar compared to Nacetylglucosamine, but some of the oligo-glucosamine with short chain oligomer could bind with a polar solvent, especially polar solvent with a higher polarity index
Summary
Chitin from the black tiger shrimp (Penaeus monodon) shell can be hydrolyzed into its monomer and oligomer. Nurrohmawati and Herdyastuti (2016) conduct a study to precipitate amorphous chitin by using ethanol and acetonitrile in the multilevel purification to produce Nacetylglucosamine. The isolation process of oligoglucosamine was done according to the principle of solubility or like dissolve like This principle shows that the polar solvent will bind the polar compound and vice versa. This research began with the enzymatic fermentation of black tiger shrimp (Penaeus monodon) chitin by using Providencia stuartii. The fermented chitin will be isolated into oligo-glucosamine or oligomer chitin with the precipitation method by using a different type of polar organic solvents and the different ratio between sample and solvent. The objectives of this research were to determine the best polar solvent and the best ratio of sample-solvent to produced oligo-glucosamine isolate with the highest concentration
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