Abstract

A gas chromatographic (GC) method has been developed for solanesol, a long-chain (C 45 terpenoid alcohol of tobacco. The method separates and quantities solanesol as its volatile trimethylsilyl derivative. After saponification of the tobacco sample with methanolic potassium hydroxide to liberate bound solanesol, total solanesol was determined on a wide-bore fused-silica SE-54 capillary column. The reproducibilities of both the extraction and GC methods were found to be excellent. As an example of application, six tobacco varieties, used in a low-solanesol tobacco breeding study, were analyzed. In these tobaccos, free solanesol content varied from 70 to 90% of the total solanesol, which ranged from 1.9 to 2.8% of dry weight.

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