Determination of Six Eugenol Residues in Aquatic Products by Gas Chromatography-Orbitrap Mass Spectrometry

Yunxia Huang,Yali Zhang,Sufang Fan,Yan Zhang,Zhijuan Meng,Xiaoxuan Yuan,Qiang Li,Yuan Liu

doi:10.1155/2021/9438853

Yunxia Huang, Yali Zhang + Show 6 more

Open Access

https://doi.org/10.1155/2021/9438853

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Abstract

Eugenol compounds are widely used in the circulation and transportation of fresh aquatic products because of their good anesthetic effects. However, some studies have shown that eugenol compounds are potential carcinogens. Therefore, in order to ensure the edible safety of aquatic products, eugenol compounds in aquatic products need to be screened quickly. A method for determination of six eugenol residues in aquatic products was established by multiplug filtration cleanup (m-PFC), combined with gas chromatography-Orbitrap mass spectrometry (Orbitrap GC-MS). Samples were ultrasonically extracted with acetonitrile, and the extracts were frozen at −18°C for 1 h, then purified with the m-PFC column, and detected by Orbitrap GC-MS in full scan mode. The results showed the linear relationships for six eugenols were good in the range of 0.001–0.1 μg/mL, and the correlation coefficients (R2) were above 0.9950. The limits of detection (LODs) were 2–10 μg/kg, and the limits of quantitation (LOQs) were 5–20 μg/kg. The average recoveries at the spiked levels of 5–200 μg/kg were in the range of 76.4%–105.1%, with relative standard deviations (RSDs) of 1.2%–7.5%. Eighty aquatic products were detected by this method, of which only eugenol was detected in 12 samples, and eugenol and isoeugenol were detected in two samples at the same time. The other eugenol compounds were not detected in any sample. The detection rate of positive samples was 17.5%. The method is simple, accurate, and suitable for the rapid screening of eugenol compounds in aquatic products.

Highlights

Eugenols are phenylpropanoid compounds, mainly including eugenol, isoeugenol, methyl eugenol, methyl isoeugenol, eugenol acetate, and acetyl isoeugenol [1]
They have the advantages of low cost, good effect, and short residual period, so they are widely used in the living transportation of aquatic products [2, 3]
In order to screen eugenol residues in aquatic products accurately and quickly, the sample was prepared by multiplug filtration cleanup (m-PFC) column with multiwalled carbon nanotubes (MWCNTs) as purification packing and detected by Orbitrap gas chromatography-mass spectrometry (GC-MS) in full scan mode through retention time and accurate molecular mass

Summary

Introduction

Mainly including eugenol, isoeugenol, methyl eugenol, methyl isoeugenol, eugenol acetate, and acetyl isoeugenol [1]. According to domestic reports in China, eugenol compounds have been detected in aquatic products, such as fish, shrimps, and crabs. Ni et al [13] determined the residues of four eugenol anesthetics in aquatic products by HLB solid-phase column combined with UHPLC-MS/MS. There are no reports on the determination of eugenol compounds in aquatic products by m-PFC column combined with Orbitrap GC-MS. In order to screen eugenol residues in aquatic products accurately and quickly, the sample was prepared by m-PFC column with MWCNTs as purification packing and detected by Orbitrap GC-MS in full scan mode through retention time and accurate molecular mass. It was the first time that m-PFC column combined with Orbitrap GCMS was applied to detect six eugenol residues in aquatic products. Provides an effective technical support for the supervision of eugenol compounds in aquatic products

Materials and Methods

Preparation of Standard Solution

Results and Discussion

Extraction Method

Method Validation