Determination of Silicon in Urine and Blood by Graphite-Furnace Atomic Absorption Spectrometry with a Nickel Chloride Modifier.

Abstract

A method has been developed for the determination of silicon in urine and blood by graphite-furnace atomic absorption spectrometry. Nickel chloride was employed for the matrix modifier. The tolerable ashing temperature was raised to 2200°C in the presence of a matrix modifier. The urine was diluted 1+349 with distilled water and the blood was diluted 1+19 with Triton X(1%), and introduced into a pyrocoated graphite tube together with a nickel solution. Recoveries of the silicon added to the urine and the blood samples ranged from 85.5 to 89.3% for urine and 69.3 to 72.0 % for blood; the relative standard deviation was within 10% at the 10 mg/l level. The silicon contents in urine and blood, determined by the proposed method, ranged from 2.6-23.7mg/l and 1.04.6 mg/l, respectively.