Abstract
Determination of sibutramine adulterated in herbal slimming product using thin layer chromatography (TLC) densitometric method with TLC silica gel 60 F 254 aluminium plate as stationary phase and mixture of toluen-diethylamine (10:0.3) as mobile phase has been developed. The calibration curve in the concentration range of 0.50 to 5.00 µg/spot showed good linier relationship (r 2 = 0.9986). The limit of detection and quantitation (LOD and LOQ) were 217.5 ng and 724.9 ng/spot, respectively. The method gave satisfactory specificity, linierity, precision and accuracy validation criteria and was applied for determination of sibutramine in herbal slimming products obtained from several drugstrores in Depok City, West Java, Indonesia. Results of the determination showed that six of seven samples analyzed were detected containing sibutramine HCl with the concentration of 2.45 - 26.24 mg in a single dosage of slimming herbal products
Highlights
Herbal medicines play an important role as an alternative of synthetic drugs to improve people health in developing countries and increasingly popular in developed countries
ABSTRAC T Determination of sibutramine adulterated in herbal slimming product using thin layer chromatography (TLC) densitometric method with TLC silica gel 60 F254 aluminium plate as stationary phase and mixture of toluen-diethylamine (10:0.3) as mobile phase has been developed
The adulteration practice violates the laws of many countries, but the presence of undeclare synthetic chemical drugs in the herbal products are still often found, including in the herbal slimming products (Departemen Kesehatan RI, 1994; World Health Organization (WHO), 2005; Carvalho et al, 2011; Badan Pengawas Obat dan Makanan (BPOM) RI, 2014; Khazan et al, 2014)
Summary
Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia 16415. ABSTRAC T Determination of sibutramine adulterated in herbal slimming product using thin layer chromatography (TLC) densitometric method with TLC silica gel 60 F254 aluminium plate as stationary phase and mixture of toluen-diethylamine (10:0.3) as mobile phase has been developed. The calibration curve in the concentration range of 0.50 to 5.00μg/spot showed good linier relationship (r2 = 0.9986). The limit of detection and quantitation (LOD and LOQ) were 217.5ng and 724.9ng/spot, respectively. The method gave satisfactory specificity, linierity, precision and accuracy validation criteria and was applied for determination of sibutramine in herbal slimming products obtained from several drugstrores in Depok City, West Java, Indonesia. Results of the determination showed that six of seven samples analyzed were detected containing sibutramine HCl w ith the concentration of 2.45-26.24mg in a single dosage of slimming herbal products
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