Determination of Short Chain Fatty Acids in Mice Feces by Capillary Electrophoresis

Letícia Marques,Mário Maróstica Junior,Cinthia Cazarin,Emanuel Carrilho,Stanislau Bogusz Junior,Juliano Bicas

doi:10.21577/0103-5053.20190031

Letícia Marques, Mário Maróstica Junior + Show 4 more

Open Access

https://doi.org/10.21577/0103-5053.20190031

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Abstract

Short-chain fatty acids (SCFA) are compounds produced during fermentation of gut microbiota. Acetic, propionic and butyric acids are the most important SCFA produced from non-digestible foods. We developed and validated a rapid and low-cost capillary electrophoretic (CE) method for determination of acetic, propionic and butyric acids in mice feces (100 mg of sample). Electrophoretic separation was performed for SCFA determination in feces samples during 10 min. The method showed good linearity for all analytes (determination coefficient, r2 > 0.98); recovery from 74.1 to 109.8%, while the intra- and interday precision essays were suitable (relative standard deviation (RSD) < 10%); and limits of detection and quantification of 0.13-0.43 mM for acetic acid, 0.09-0.29 mM for propionic acid and 0.03-0.09 mM for butyric acid. Real samples of mice dextran sulfate sodium-induced colitis feces treated with jabuticaba (Plinia cauliflora) aqueous extract were performed successfully.

Highlights

Short-chain fatty acids (SCFA) called as volatile fatty acids are defined as saturated aliphatic organic compounds containing between 2 to 6 carbon atoms
The separation of SCFA depends on their electrophoretic mobility, which is proportional to the charge of the ion and inversely proportional to the molecular mass, shape of the molecule
The obtained electropherograms for SCFA analysis were quite clear of interferences, showing up basically signals from the analytes and electroosmotic flow (EOF) marker with good resolution

Summary

Introduction

Short-chain fatty acids (SCFA) called as volatile fatty acids are defined as saturated aliphatic organic compounds containing between 2 to 6 carbon atoms. SCFA are involved directly or indirectly in many physiologic responses correlated to the immunologic processes, anti-carcinogenic action and cardiovascular diseases risk-reducing.[7,8,9] Anti-inflammatory effects of SCFA can be observed due to reducing of signaling of Acetic acid (C2), propionic acid (C3), and butyric acid (C4) are regarded as the most important SCFA to human health. Their abundance is about 95% of all SCFA produced and the ratio acetate:propionate:butyrate (about 57:22:21) is basically the same at distinct regions of the large intestine.

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