Determination of seven flavonoids in Qingre Zhike Granules and intermediate product with quantitative analysis of multi-components by single marker

Abstract

This study is to determine seven flavonoids( narirutin,naringin,hesperidin,neohesperidin,baicalin,oroxylin A-7-glucoronide and wogonoside) in Qingre Zhike Granules and intermediate products by quantitative analysis of multi-components with a single marker( QAMS). High performance liquid chromatography( HPLC) was performed on Thermo BETASIL C18 chromatographic column( 4.6 mm×250 mm,5 μm) with acetonitrile-0.1% formic acid solution as mobile phase for gradient elution at a flow rate of 0. 8 m L·min-1. The detection wavelength was set at 280 nm and the column temperature was 30 ℃. Six relative correction factors( RCFs)of narirutin,naringin,hesperidin,baicalin,oroxylin A-7-glucoronide and wogonoside were established in the HPLC method with neohesperidin as the internal standard,and then these RCFs were used to calculate the mass fractions of the six components. Such mass fractions were compared with the mass fraction determined by the external standard method( ESM) at the same time to verify the feasibility and accuracy of QAMS method. Within the linear range,the RCFs of narirutin,naringin,hesperidin,baicalin,oroxylin A-7-glucoronide and wogonoside were 1.150,1.109,1.110,0.658,0.723 and 0.572,respectively,with good repeatability in different experimental conditions. There was no significant difference between the QAMS method and ESM method. The QAMS method with neohesperidin as the internal standard is feasible and accurate for the determination of narirutin,naringin,hesperidin,baicalin,oroxylin A-7-glucoronide and wogonoside,and it can be used for quality control of Qingre Zhike Granules and intermediate products.