Abstract
Serum ionised calcium concentration [Ca 2+] was measured with a calcium selective electrode in 65 normal people. A mean value of 1.06 mmol/1 (± 0.04 S.D.) with an actual range of 0.97 to 1.13 mmol/1 was obtained. Serum samples refrigerated at 4° C for 24 h were satisfactory for analysis. Storage of whole blood samples for 6 h at room temperature before separation caused a very small error (+0.04 mmol/1). Mean results and ranges were similar in males and females and there was no significant correlation with age. No significant change in serum [Ca 2+] was found following a normal meal. Serum [Ca 2+] and total serum calcium showed a very slight correlation ( r = 0.35). The method is reproducible and sensitive.
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