Determination of serum glutamate oxaloacetate transaminase and glutamate pyruvate transaminase by using L-glutamate oxidase.

Abstract

Serum glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities were determined by using L-glutamate oxidase from Streptomyces violascens. GOT and GPT produce L-glutamic acid as a common product, and L-glutamic acid can be easily determined by using L-glutamate oxidase. Hydrogen peroxide produced from L-glutamic acid by L-glutamate oxidase was consumed to coupled two mol of homovanillic acid to yield the fluorophore in the presence of peroxidase. The resulting fluorophore was determined with a spectrofluorometer at an excitation wavelength of 315 nm and an emission wavelength of 425 nm. The GOT and GPT activities obtained by our method were well correlated with those obtained by the Centrifichem System 400 method.