Abstract

Different types of nitrocellulose membranes filters, viz. Snypor, Sartorius and Millipore, were tested as a carrier medium for chromatography and electrophoresis of proteins. All the membranes displayed a characteristics strong adsorptivity towards proteins which could be prevented by an impregnation with neutral detergents ( e.g. the Tweens). Some important differences in chromatography on various types of membranes were also found and discussed. Rapid quantitative protein determinations could be obtained by radial membrane chromatography of solutions containing concentrations of 0.001–6 % of various proteins.

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