Abstract
Abstract Serum chemistry analysis is used for the evaluation of a subject's health status in reptile medicine. Protein electrophoresis, combined with the determination of total protein concentration, is the standard method for the determination of serum albumin concentration. An alternative method is the bromocresol green (BCG) dye-binding method. The BCG method for the measurement of albumin concentration in reptiles may lead to inaccurate results for serum albumin concentration. The aim of this study was to compare the serum albumin values of Hermann's tortoises (Testudo hermanni) obtained by protein electrophoresis and the BCG method and to test the reliability of the BCG method for the measurement of albumin concentration in this species of tortoise. Between May and June 2012, serum samples from 32 Hermann's tortoises, 22 healthy and 10 diseased, were analyzed. Serum albumin concentrations were measured by protein electrophoresis and by the BCG method. The results of the two methods were compared usin...
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