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Abstract
The sedative compounds in two traditional Chinese medicines were studied. These compounds are present in the medicinal herbs “Zao Ren” and “Lu Lu Tong”. The samples were first extracted by ethanol and pre-treated by ultrasonic wave, and then they were separated and qualitatively analyzed by high-resolution GC-EI-MS and High Performance Liquid Chromatography (HPLC) systems. We have found that the Chinese herb medicines “Zao Ren” contained clonazepam (120 ppm), whereas “Lu Lu Tong” Chinese drug contained estazolam (270 ppm). The Analytical measurements were done by using three different diagnostic indices using the GC-retention time of the GC-EI-MS total –ion–chromatogram; single stage EI-MS analysis identification and from the measurements of the retention time in the HPLC.
Highlights
In the world, many people are suffering from neurological diseases which cause serious anxiety, insomnia, convulsions, and epilepsy [1]
The samples were first extracted by ethanol and pre-treated by ultrasonic wave, and they were separated and qualitatively analyzed by high-resolution GC-EI-MS and High Performance Liquid Chromatography (HPLC) systems
We have found that the Chinese herb medicines “Zao Ren” contained clonazepam (120 ppm), whereas “Lu Lu Tong” Chinese drug contained estazolam (270 ppm)
Summary
Many people are suffering from neurological diseases which cause serious anxiety, insomnia, convulsions, and epilepsy [1] These patients often need drugs to relieve their symptom. Western medicine have used in the last century natural product extracts and chemical synthesized derivative drugs for treating various diseases. This technology has evolved to derive from and possessing a certain composition and mature analysis methods, is wisely used. We would like to try to understand and define the treatment mechanism of Chinese TCM. In this rationale we are reporting our efforts on the analysis of the TCM component. After a series of analytical experimental studies, we have established a method for analyzing and several effective components of TCM
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