Abstract
An analysis and detection method for eight sedatives and fifteen β-receptor hormones in feed was established by ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The sample was extracted with acetonitrile-1% (v/v) trichloroacetic acid aqueous solution (7:3, v/v), and purified by cationic solid phase extraction column. The extracts were separated on an Agilent Zorbax Eclipse Plus C18 chromatographic column (100 mm×3.0 mm, 1.8 μm) and then quantified via internal standard method. The results showed that the linear relationships of the 23 targets were good in the range of 2.0-200.0 μg/L (r2> 0.99). The average recoveries of the targets spiked in the matrix of feed samples were within 75.1%-102.4% in three spiked levels of 5.0, 10, 50 μg/kg, and the relative standard deviations (RSDs) were within 4.3%-14.3% (n=6). This method with high purification efficiency and extensive scope of application can be applied to screening and detecting sedatives and β -receptor hormones in feed.
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