Determination of seco-iridoid and 4-pyrone compounds in hydro-alcoholic extracts of <i>Gentiana lutea</i> L. subsp. <i>symphyandra</i> Murb. leaves and roots by using high performance liquid chromatography

Abstract

In view of the increasing interest in herbal remedies and their important role in health maintenance and well-being, a study was carried out to clarify the role and importance of the solvent in the extraction process. Hydro-alcoholic (methanol or ethanol) or water extraction procedures for secondary metabolites from Gentiana lutea subsp. symphyandra leaves and roots were optimized for the yield of compounds that have proved to act antioxidatively. Using high performance liquid chromatography (HPLC), the contents of seco-iridoid glucosides (gentiopicroside, amarogentin) and 4-pyrones (mangiferin (xanthone), homoorientin (flavone C-glycoside), isovitexin (flavone C-glycoside)) in Gentiana lutea subsp. symphyandra extracts were determined. The analysis revealed that the contents of observed secondary metabolites in Gentiana lutea subsp. symphyandra extracts varied significantly based on the solvent (methanol, ethanol, and water) used in the extraction procedure. Seco-iridoids and 4-pyrone contents in extract...