Abstract
A method of the quantitative determination of saccharin in foods by gas liquid chromatography (GLC) was described.Saccharin was methylated with dimethylformamide dimethylacetal (DMF-Me) heating for 30min in glycerin bath at 110±5°C. After reaction with DMF-Me, methyl derivative of saccharin obtained was determined by GLC equipped with a flame ionization detector (FID) and an electron capture detector (ECD). GLC was performed using DC-200 (10%) on gas chrom Q as the column and β-BHC as an internal standard.The recoveries of sodium saccharin added in various foods by the present method were 88.1-94.7%, and the limit of detection as saccharin was about 0.02μg with FID-GLC and 0.02ng with ECD-GLC.Furthermore, the determination with ECD-GLC was an effective method for the ultramicro-analysis.
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