Abstract
A sensitive and selective high performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method was developed and validated for the determination of rocuronium in human plasma. The plasma samples were separated on an Agilent SB-C18 column (150×2.1 mm, 3.5 μm) with a mobile phase consisted of 20 mM ammonium acetate/methanol/ acetonitrile (20/40/40, v/v/v) at a flow rate of 0.7 mL·min-1. Electrospray ionization (ESI) source was applied and operated in the positive ion mode. Multiple Reaction Monitoring (MRM) modes with the transitions of m/z 529.3→487.3 (rocuronium) and 321.0→275.0 (IS) was used. A good linearity was obtained in the concentration range of 5~3000 μg·L-1 (r=0.9976, n=5). The inter- and intra-day precision (RSD) were less than 8.1%. The extraction recoveries were 92.0~92.6%. Rocuronium in plasma was stable when frozen at -20oC for 24 hours and seven days, and also stable after two freeze-thawing cycles. The method is simple, quick, sensitive, reproducible and can be used for the pharmacokinetic and bioequivalence study of rocuronium.
Highlights
Rocuronium, a new type of muscle relaxant with the features of single quaternary ammonium steroids, medium aging and non depolarizing, was used for anesthesia and surgery of endotracheal intubation of muscle relaxation, which has quick effect, short duration and no cumulative effect, and do not produce tachycardia and blood pressure change, no histamine release, and other features
Farenc et al [5] established a high performance liquid chromatographymass spectrometric (LC-MS) method to determine the concentration of rocuronium in human plasma
The Electrospray ionization (ESI) source was set to positive ion mode and multiple reaction monitoring (MRM) mode was used to detect rocuronium and lorazepam at the transition of 529.3→487.3 and 321.0→275.0, with spray gas pressure of 40 psi, protective air of nitrogen gas at a flow rate of 9 L/min, capillary voltage of 4000 V, fragment electric voltage of 100 V, and collision energy of 45 eV for rocuroniumand 20 eV for I.S
Summary
Rocuronium, a new type of muscle relaxant with the features of single quaternary ammonium steroids, medium aging and non depolarizing, was used for anesthesia and surgery of endotracheal intubation of muscle relaxation, which has quick effect, short duration and no cumulative effect, and do not produce tachycardia and blood pressure change, no histamine release, and other features. Less studies were reported on the determination of rocuronium. Yu et al [3] reported a gas chromatography mass spectrometric (GCMS) method for determination rocuronium in human plasma, which has higher limit of quantitation and longer time for sample analysis.
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