Abstract

The fluorimetric determination of riboflavin by an automated procedure is described. The procedure allows up to thirty samples plus standards and blanks to be analysed in each run. After measurement, sodium dithionite treatment is used to eliminate the riboflavin fluorescence, and a second reading makes it possible to calculate net fluorescence values which can be related with concentration of riboflavin in the original samples. Linear response to riboflavin is obtained over the range 0–25 μg ml -1. Repeatability is satisfactory for content uniformity and assay tests of riboflavin in oral pharmaceutical dosages.

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