Abstract
Abstract A procedure is presented for the determination of reserpine, hydralazine HCI, and hydrochlorothiazide in tablets by liquid chromatography. The sample is extracted with methanol, and the extract is filtered through paper. Chromatography is performed in 2 stages, each using a 7.5 cm-long normal-phase column but different mobile phases. In the first stage, intended exclusively for reserpine, the mobile phase consists of methanol containing 2% aqueous 1-pentanesulfonic acid sodium salt at 4 parts/1000. Detection and quantitation of reserpine was by fluorescence at 280 nm (excitation) and 360 nm (emission). In the second stage, the amount of the salt solution in the mobile phase was increased to 5%. Detection and quantitation of hydrochlorothiazide and hydralazine HCI was by UV absorbance at 260 nm. One commercial sample of tablets was analyzed by the proposed method. Two determination of each ingredient were made on a ground composite. Ten individual tablets also were examined.
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