Abstract
SummaryThe ascorbic acid value of blood is materially affected by standing either in the ice-box or at room temperature. Determinations should be carried out within one-half hour after the collection of the blood.Since the appearance of the clinical methods for determining blood ascorbic acid by Farmer and Abt1,2 considerable interest has been aroused in the study of vitamin C in various diseased conditions. The determination depends on the following proceduure: Deproteinization of the plasma by 10% metaphoric acid3 and titration of the reduced ascorbic acid present in the filtrate with 2:6 dichlorophenol indophenol.4By the use of this method we have obtained high and low ascorbic acid values in presumably normal subjects, or at least in individuals in whom there was no reason to suspect vitamin C deficiency. Furthermore, determinations on the same subject within 2 or 3 hours yielded various blood ascorbic acid values. In all these determinations great care was exercised to avoid the presence of disturbin...
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