Abstract
A new quantitative densitometric high-performance thin-layer chromatographic (HPTLC) method was developed and validated for the analysis of ranolazine (RZ) both in bulk and formulations. RZ was separated and identified on silica gel 60 F254 HPTLC plates with butanol—acetic acid—water (6:2:2 v/v) as the mobile phase. Densitometric quantification was performed at λ = 270 nm by reflectance scanning which facilitated well-resolved band for the main drug (RF 0.56 ± 0.02). Response to RZ was a linear function of concentration in the range of 100–400 ng, with a correlation coefficient, slope, and intercept of 0.99912 ± 0.00017, 8.684 ± 0.582, and 492.147 ± 2.67, respectively. The minimum amount of RZ that could be authentically detected and quantified was 14.90 and 49.67 ng band−1, respectively. Additionally, the peak identities as well as the purities were confirmed by mass spectrometry. The electrospray ionization (ESI+) mass spectra showed the [M + H]+ ion for RZ detected at m/z 428.3 being acquired directly ...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: JPC - Journal of Planar Chromatography - Modern TLC
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
