Abstract
An anion exchange chromatographic method has been developed to quantify raffinose and lactobionic acid in ViaSpan ®, an organ preservation product. Separation was accomplished using an aqueous sodium hydroxide/acetate solvent system (pH 13) on a Carbopac PA1 column. Detection was performed using a pulsed amperometric detector equipped with a gold working electrode. The method was able to resolve raffinose, lactobionic acid and other ingredients in the ViaSpan ® product. Validation testing of the method for routine use produced excellent linearity, precision and accuracy. The limits of detection for raffinose and lactobionic acid were 1.0 ng (1.7 × 10 −12 mol, S/ N = 3) and 2.0 ng (5.6 × 10 −12 mol, S/ N = 3) respectively. The total analysis time is less than 15 min.
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