Abstract
An anion exchange chromatographic method has been developed to quantify raffinose and lactobionic acid in ViaSpan ®, an organ preservation product. Separation was accomplished using an aqueous sodium hydroxide/acetate solvent system (pH 13) on a Carbopac PA1 column. Detection was performed using a pulsed amperometric detector equipped with a gold working electrode. The method was able to resolve raffinose, lactobionic acid and other ingredients in the ViaSpan ® product. Validation testing of the method for routine use produced excellent linearity, precision and accuracy. The limits of detection for raffinose and lactobionic acid were 1.0 ng (1.7 × 10 −12 mol, S/ N = 3) and 2.0 ng (5.6 × 10 −12 mol, S/ N = 3) respectively. The total analysis time is less than 15 min.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
