Abstract
To establish a high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for simultaneous determination of olaquindox, quinoxaline-2-carboxylic acid, 3-methyl-quinoxaline-2-carboxylic acid in food products of animal origin. Samples were extracted with ethyl acetate-0.1 mol/L sodium dihydrogen phosphate(1∶9, V/V) solution in water bath at 40 ℃, purified by solid phase extraction column(olaquindox uses HLB SPE cartridge, quinoxaline-2-carboxylic acid and 3-methyl-quinoxaline-2-carboxylic acid use MAX SPE cartridge), evaporated by concentrator, and dissolved by mobile phase. The supernatant was separated on Agilent ZORBAX SB-C_(18) column(2.1 mm×50 mm, 1.8 m), using 0.2% formic acid solution methanol solution as mobile phase, and then detected by HPLC-MS/MS using multiple reaction monitoring(MRM) in positive ionization mode. The linear range for olaquindox、quinoxaline-2-carboxylic acid and 3-methyl-quinoxaline-2-carboxylic acid were 2.5-100 μg/L, r & gt; 0.9990.The average recoveries were 72.6%-90.5% and the precisions were 3.2%-13.1%(n=6). The detection limits for olaquindox、quinoxaline-2-carboxylic acid and 3-methyl-quinoxaline-2-carboxylic acid were 0.08 μg/kg. The method is specific, sensitive, easy, fast and suitable for the confirmation and quantification of olaquindox、quinoxaline-2-carboxylic acid、3-methyl-quinoxaline-2-carboxylic acid in food products of animal origin.
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