Determination of Punicalagins Content, Metal Chelating, and Antioxidant Properties of Edible Pomegranate (Punica granatum L) Peels and Seeds Grown in Morocco.

Talal Sabraoui,Rabiaa Eddoha,Maryam Benbachir,Taleb Khider,Abderrahman Moujahid,Boubker Nasser,Abdelkhalid Essamadi

doi:10.1155/2020/8885889

Talal Sabraoui, Rabiaa Eddoha + Show 5 more

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https://doi.org/10.1155/2020/8885889

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Abstract

Pomegranate (Punica granatum L) is widely cultivated in the Mediterranean countries especially in Morocco. Pomegranate peel and seed contain considerable amounts of phenolic compounds with antioxidant activity. The aim of the present study was to phytochemically characterize the pomegranate peels and seeds obtained from three Moroccan provinces, using UHPLC-DAD. In addition, total phenolic content (TPC), total flavonoid contents (TFC), and metal chelating of pomegranate peel were also evaluated. The results showed that pomegranate peel possesses the highest phenolic (TPC: 224.39 mg GAE/g dw) and flavonoid (TFC: 62.64 mg rutin/g dw) contents. Punicalagin-β and punicalagin-α, are the abundant compounds found in peel: 216.36 ± 9.94 mg/g, 154.94 ± 5.21 mg/g, respectively. Pomegranate peels showed significantly (p < 0.05) high antioxidant activity 1-diphenyl-2-picrylhydrazyl (DPPH) EC50: 42.71 ± 0.04 μg/mL, 2.2′-Azino-bis(3-Ethylbenzothiazoline-6-Sulfonic Acid) (ABTS) EC50: 62.15 ± 0.01 μg/mL), and chelating activity (FRAP 1.85 ± 0.00 mg ascorbic acid equivalents/100 g, Fe2+: 2.52 ± 0.01 μmol EDTA equivalents/g dw) compared to seeds. A positive correlation between antioxidant activity and total phenolic was found. According to achieved results, high antioxidant capacity of pomegranate extracts, especially peel, shed light to further use as natural food preservatives. Pomegranate peel could be used for the fortification of food with fiber by introducing it in dietary, as well as in health applications due to its higher antioxidant capacity.

Highlights

The concentrations of total phenolic content found in Beni Mellal, Berkane, and Settat peel were 3.52, 3.29, and 3.30-folds higher than the content determined in seeds, respectively (Table 1)
Khalil et al [36] studied the punicalagin contents and antioxidant activity after juice extraction obtaining a phenolic content of 289.40 mg gallic acid equivalents (GAE)/g dw in peel methanolic extract
Seed phenolic compounds were evaluated between 62.17 and 67.86 mg GAE/g dw, consistent with the data reported by Orak et al [11] where the phenolic compounds of seed using methanol as a solvent ranged from 54.48 to 63.43 mg GAE/g

Summary

Introduction

L) is a highly appreciated edible fruit tree in the world. The source of pomegranate is related to central Asia, from Iran to China and Eastern India [1]. The cultivation of the fruit has spread to Mediterranean countries like Turkey and North Africa. The commercial cultivation of pomegranate is mainly concentrated in the Mediterranean countries followed by Asian countries [2]. Different cultures as ancient Egyptians and Indians used pomegranate in traditional medicine for many centuries [3]

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