Abstract
Molecular absorption in an enhanced resonance light scattering (RLS) system decreases the enhanced RLS signals detected by using a common spectrofluorometer, lowering the detection sensitivity of RLS method. Thus a mathematical compensation for the decreased RLS signals resulting from the molecular absorption in the detection system is necessary so as to improve the detection sensitivity. Starting from Beer's law, herein we propose a compensation method by calculating a compensation factor related to the transmittance after the incident beam has passed through the absorption system. The compensation factor can be measured with an equipped accessory of the spectrofluorometer introducing a cell holder to change the propagation direction of the excitation light beam of the spectrofluorometer. By taking the interaction of Ponceau G (PG) with proteins as an example, we proved that the present compensation for the molecular absorption-decreased RLS signals could improve the detection sensitivity about two folds.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
