Abstract
ABSTRACT A novel assay of proteins with the determination sensitivity at nanogram levels has been set up by using a common spectrofluorometer to detect intensity of resonance light scattering (RLS). In an acid aqueous medium, the interactions of Sodium dodecyl benzene sulfonate (SDBS) with different proteins can result in obviously enhanced RLS signals. With the enhanced RLS signals at 470.0 nm, proteins, including bovine serum albumin (BSA), human serum albumin (HSA), α-chymotrypsin (Chy), lysozyme (Lys), can be determined with the limit of detection below 58.1 ng/ml. Synthetic samples and human urine samples were analyzed satisfactorily.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
