Abstract
The determination of Prostaglandin (PG) E1 in plasma is challenged by its low concentration (pg/mL) and endogenous interference. An LC-MS/MS method for the determination of PGE1 in dog plasma has been developed and validated. Plasma being sampled at 4°C and treated with indomethacin effectively inhibited interferents synthesized post-sampling. Samples were subjected to one-step extraction and separated by reversed phase HPLC with a short cycle time of 3min. An LLOQ of 10pg/mL was achieved with 500μl plasma. The method was applied to a pharmacokinetic study in beagle dogs involving an intravenous infusion of 3.2μg/kg PGE1. The half-life was recovered at 7min. The simple, sensitive and rapid method was suitable to be applied to pharmacokinetic studies of PGE1 at clinically relevant doses.
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