Abstract
Polyamines not only affect transcription and translation but also may induce a number of posttranslational modifications. The identification of polyamine-induced posttranslational modifications can be performed by 2D PAGE analyses. Here, we provide a protocol for 2D-gel electrophoresis that has been optimized for plants. The combined use of this protocol with epitope-tagged proteins expressed in plants enables the detailed analysis of posttranslational modifications induced by different polyamines in vivo.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Published version (
Free)
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
