Abstract
A high-performance liquid chromatographic (HPLC) method is described for determining the polymer, dimer and monomer distribution in commercial albumin preparations. This method is a satisfactory substitute for conventional gel chromatography. HPLC can also be used to purify the albumin monomer from partially purified preparations. All fractions (monomer, dimer and polymer) still showed the presence of impurities when sensitive analysis by crossed immunoelectrophoresis was used, so that accurate extinction coefficients for protein determination cannot be assigned to these fractions.
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