Abstract
High performance liquid chromatography was applied to the separation and determination of polycyclic aromatic hydrocarbons (PAH) extracted from diesel particulates. Soluble organic fraction (SOF) contained in PAH was obtained by Soxhlet extraction of particulate samples on filters. PAH were selectively separated from SOF samples, by a silica cartridge with solvents and they were then used for HPLC analyses. Each PAH was separated and quantified by using the methods of reversed phase and partition chromatography with gradient elution. Fluorescence detection with variable excitation and fluorescence wavelength was applied to detect many kinds of PAH with detection limits from 0.24 to 0.91 μg l-1. It was found that aromatics in the fuel strongly affected the quantity of PAH in the SOF.
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