Year-end Sale % OFF 
Abstract
The polyamines spermine, spermidine, putrescine and cadaverine were treated with o-phthalaldehyde and ethanethiol. After reaction time of 90 sec., the stability of the derivatives was examined in the reaction medium, in an ethyl acetate extract and in different mobile phases used for high-performance liquid chromatography on reversed phase columns. The stability of the ethanethiol derivatives was improved compared to the 2-mercaptoethanol derivatives. Increasing concentrations of alcohol in the reaction medium increased the stability, but a significantly higher stability was obtained by including a simple and rapid extraction with ethyl acetate. With a mobile phase of N,N-dimethylcyclohexylamine (0.1 M) and phosphoric acid (0.2 M) in a 70–90% methanol gradient, decomposition on the column was minimized and a satisfactory resoluton was obtained. A detection limit of less than 0.5 pmol of each polyamine was obtained. by fluorescence detection.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
