Abstract
Objective To establish a method to determine warfarin concentration in plasma by ultra performance liquid chromatography (UPLC), and to explore the relationship between warfarin plasma concentration and international normalized ratio (INR) in patients after cardiac valve replacement, so as to seek more safe and reliable monitoring indicators and guide rational use of anticoagulant therapy. Methods An ultra performance liquid chromatography method has been developed for measuring warfarin concentration in plasma by using 6-methoxy naphthaleneacetic acid (6-MNA) as internal standard (IS). At the some time, the correlation of INR, warfarin dosage and plasma warfarin concentration in 79 samples was analyzed. Results The retention time of warfarin and the IS were 2.2 and 1.1 min, respectively. The average extraction recovery of plasma warfarin was 96.0%, The concentration of warfarin was 15.6~4000 ng/ml with good linearity (R2=0.9996). The overall accuracy of the method was 98.5%~99.9% and the lowest detection limit was 5.0 ng/ml. The intra-and inter-day variations were less than 1.34% and 2.69%, respectively. The correlation between INR and warfarin dose was r2=0.006 (P=0.481), INR and plasma warfarin concentration was r2=0.006 (P=0.497), and the correlation between warfarin dose and plasma concentration was r2=0.298 (P=0.001) in 79 cases of samples. Conclusions A simple, rapid, accurate and sensitive UPLC method for quantifying warfarin levels in human plasma was validated. The determination of warfarin blood concentration has guiding significance for anticoagulant therapy of patients after heart valve replacement. Key words: Warfarin; International normalized ratio; Ultra performance liquid chromatography
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
