Abstract
An agar gel radial diffusion assay for proteinases has been adapted for the assay of proteinase inhibitors from plants. It requires minimal equipment and produces results comparable to those obtainable with a conventional spectrophotometric technique (TAME assay). Insect gut proteinase preparations can be employed in the assay, as well as bovine trypsin. The assay should be useful in large-scale surveys of plant materials for inhibitor activity. Using it, we demonstrate that Jimson weed foliage contains a heat-labile inhibitor of bovine trypsin whose levels rise markedly in undamaged leaves 72 h after crushing injury to the cotyledons or leaves of the plant.
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