Determination of Plant Constituents in Fractions of Cordia obliqua Willd. Leaf Methanol Extract using GC-MS Analysis

Abstract

Cordia obliqua Willd. is an important medicinal plant of family Boraginaceae. It is commonly known as Clammy Cherry. It is a deciduous tree and found throughout Himalayan region in India. Traditionally, it possesses a number of medicinal activities like diuretic, expectorant, antipyretic, anthelmintic and maturant. The current work is aimed to investigate chemical constituents present in fractions of leaf methanol extract of Cordia obliqua plant using GC-MS analysis. Two fractions of methanol extract (CO1 and CO3) were studied and about 30 components were detected in each sample. Highest peak area was observed for moncyclic sesquiterpene alcohol α-Bisabolol oxide b at RT 20.84 in CO1 and for o-methyl-d-glucose at RT 16.53 in CO3. The important constituents detected were Amino acids, Oleic acid, Oleanolic acid, Sesquiterpene lactone, trietrpenoids, various steroids, galactopyranoside, Hexadecanoic acid, Phthalic acid, Azafrin, Decatrienoic acid, Docasenamide, Morphinan and carotenoids. Among these Morphinan is an opoid alkaloid and good psychoactive drug. Other constituents are also medicinally important and many have reported biological activities. Thus GC-MS analysis revealed the existence of various types of constituents in Cordia obliqua leaf methanol extract fraction which confirm the application of this plant for a number of medicinal activities.