Abstract
Three common genetically classified placental alkaline phosphatase variants (F, I, S) were purified to complete homogeneity. Specific antisera were raised against all three types. Complete fusion reactions in double diffusion tests were obtained for all combinations of antigen and antibody, indicating close immunological relatedness.A sensitive and specific double antibody solid phase (DASP) radioimmunoassay with a least detectable dose of 9 ng/ml was developed.Sera from 241 patients with different malignant disorders were assayed for the presence of placental alkaline phosphatase, also called the Regan isoenzyme. Significantly elevated values were obtained in only one case of embryonal carcinoma. This corresponds to a frequency of 0.4%. Three selected sera from cancer patients with a known content of Regan isoenzyme were tested in the same assay and had elevated values.
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