Determination of Phytochemicals, in vitro Antioxidant and Antibacterial activity of Lavandula angustifolia Mill.

Abstract

The aim of the current study was to screen out the phytochemical constituents viz., alkaloids, flavonoids, terpenoids, steroids, tannins, anthraquinones, phenols, carbohydrates, proteins, saponins and glycosides from the leaf extracts of Lavandula angustifolia by quantification method. Further, the evaluation of antioxidant properties was carried out by DPHH assay by using methanol extract while as antibacterial assay was done with aqueous and methanolic extracts. Phytochemical screening of L. angustifolia leaf extract exhibited the existence of alkaloids, flavonoids, steroids, tannins, anthraquinones, phenols, carbohydrates, proteins and glycosides. The protein, lipid, flavonoid, carbohydrate, phenolic and tannin content was estimated in methanolic extract and it was found proteins, lipids and flavonoids were present in highest concentration followed by carbohydrates, phenols and tannins. In the DPHH radical scavenging assay, the methanolic leaf extracts of the L. angustifolia scavenged 63.51±1.43% of DPHH radicals at the concentration of 250µg/ml. The result was compared with the ascorbic acid as standard molecule as it showed 95.57±1.12 of DPHH radical scavenging activity at 80 µg/ml. The present investigation showed the L. angustifolia extracts possess the significant quantities of phytochemical constituent and also exhibited good antioxidant as well as antibacterial activity. The results demonstrated that the antioxidant capacity exhibited in the methanolic leaf extracts of L. angustifolia would be due to the presence of phenolic components and our outcomes validated its use in traditional medicine.