Determination of phytochemical profile and antioxidant efficacy of Lavendula bipinnata leaves collected during Magha Nakshatra days and Normal days using LC-QTOF-MS technique

Abstract

In the present work, we determined the phytochemical and antioxidant potential of Lavendula bipinnata leaves collected during Magha Nakshatra (NAK-leaf) and Normal (NOR-leaf) days. The extraction was done using decoction and maceration (DCE and MCE) and microwave assisted extraction and ultra sonic assisted extraction (MAE and UAE) methods and in all 16 extracts were obtained. Total phenol, flavonoid, flavonol, phenolic acid and proanthocyanidin content was estimated in all the extracts. Antioxidant efficacy of all the extracts was assessed by four different in vitro antioxidant assays viz. DPPH, super oxide, ABTS radical scavenging activity and ferric reducing antioxidant power. The obtained results revealed that MAE extracts of NAK-leaf showed better phytochemical content and antioxidant activity which was better than the standards revealing its excellent antioxidant potential. Pearson correlation analysis revealed that phenolic and non-phenolic compounds were responsible for the antioxidant activity. LC-QTOF-MS based metabolic profiling of MAE extract revealed 35 compounds in NAK- leaf and 33 compounds in NOR-leaf; their structure was determined and many of them were pharmacologically active. Hence, it can be concluded that the leaves of Lavendula bipinnata can be a good natural source of antioxidant compounds which can definitely be used in drug designing to fight against oxidative stress related diseases.