Determination of Phytochemical Content by Chromatographic Methods and Antioxidant Capacity in Methanolic Extract of Jujube (Zizyphus jujuba Mill.) and Oleaster (Elaeagnus angustifolia L.)

Abstract

ABSTRACT In this study, the antioxidant effects, protein contents and phytochemical structure content of jujube, oleaster fruits and their seeds were investigated. Methanol extraction of fruits and seeds was prepared and volatile organic components were examined by GC-MS and phenolic components were examined by LC-MS/MS. Antioxidant activity was determined by DPPH and NO scavenging (%). In addition, mineral content was defined with ICP-MS. DPPH scavenging activity (%) was observed most in jujube fruit (80.36%) then it continued as oleaster seed (79.33%) and oleaster fruit (72.00%) and jujube seed (66.26%) respectively. According to protein results, there was a 2.19-fold difference between jujube fruit and seed. This difference was found 0.78 times between oleaster fruit and seed. 4-hydroxybenzoic acid, gallic acid and vanillic acid contents of phenolic compounds were found in high amounts. P (phosphorus) contents in jujube, oleaster, jujube seed and oleaster seed, respectively; 722.67, 736.91, 453.74 and 687.10 mg/kg. In the jujube and jujube seed samples, the contents of Ca (calcium) were found 1261.02 and 2228.24 mg/kg. K (potassium) contents were determined 7351.16 and 7688.42 mg/kg in jujube and oleaster, respectively.