Abstract
Biological tissues are digested with nitric and perchloric acids in a heated aluminium block. Flow-injection spectrophotometry is then used to measure phosphate via the phosphovanadomolybdate complex at 413 nm. The detection limit is 0.3 μg ml −1 phosphorus; relative standard deviations are 0.7% and 0.4% at 1 μg ml −1 and 25 μg ml −1 phosphorus, respectively. Interferences are discussed. The decomposition procedure is evaluated for model compounds and standard reference materials.
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