Abstract
Phospholipids (PLs), the most important class of polar lipids in foods, are ubiquitous compounds because they are the major components of animal and plant cell membranes. Their technological and biological properties therefore make them important in human nutrition. The efficient separation and accurate quantification of PLs can be achieved with high-performance liquid chromatography–evaporative light scattering detection (HPLC-ELSD) because ELSD is a quasi-universal detector for liquid, countercurrent, and supercritical fluid chromatography and can detect any analyte less volatile than the mobile phase. In this article, the principles of ELSD operation (i.e., nebulization of the chromatographic effluent, evaporation of the mobile phase, and measurement of the scattered light) as well as the application of LC-ELSD to determine the amount of PLs in different food matrices are reviewed. Food matrices containing PLs include milk and dairy products, meat, fish, eggs, cereals, and oils. Both the technological aspects and analytical parameters affecting the concentration and quantitative determination of PLs in food matrices are evaluated. In particular, different extraction, purification, and chromatographic conditions were extensively investigated; moreover, wherever possible, the results obtained are reported and compared with other detection methods.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
