Abstract
Penicillin and the corresponding penicilloic acid are extracted by chloroform from an acidified sample of urine which has been partly saturated by ammonium sulfate. A portion of the extract is dried and redissolved in a small volume of acetone. A measured aliquot is chromatographed on a silica gel thin-layer plate by acetone-acetic acid (19:1). The separated penicilloic acid is detected by starch-iodine spraying. The zones are transferred into glass tubes, nitrated, and neutralized by ammonia. The absorbance of the yellow supernatant, which is proportional to the concentration of penicilloic acid, is measured spectrophtometrically.
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